期刊
DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
卷 109, 期 -, 页码 -出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.dci.2020.103701
关键词
Apis mellifera; Monoclonal antibody; Immunity; Hemocyte; Honey bee; Insect immunity
资金
- Swedish Research Council
- Hungarian National Beekeepers Association
- [NKFI K 120140]
- [NKFI K 120142]
- [GINOP-2.3.2-15-2016-00001]
- [GINOP-2.3.2-15-2016-00035]
- [NKFI K 128762]
- [NKFI K 131484]
- [EFOP-3.6.3-VEKOP-16-2017-00008]
Cell mediated immunity of the honey bee (Apis mellifera) involves the activity of several hemocyte populations, currently defined by morphological features and lectin binding characteristics. The objective of the present study was to identify molecular markers capable of characterizing subsets of honey bee hemocytes. We developed and employed monoclonal antibodies with restricted reactions to functionally distinct hemocyte subpopulations. Melanizing cells, known as oenocytoids, were defined by an antibody to prophenoloxidase, aggregating cells were identified by the expression of Hemolectin, and phagocytic cells were identified by a marker expressed on granulocytes. We anticipate that this combination of antibodies not only allows for the detection of functionally distinct hemocyte subtypes, but will help to further the exploration of hematopoietic compartments, as well as reveal details of the honey bee cellular immune defense against parasites and microbes.
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