期刊
DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
卷 114, 期 -, 页码 -出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.dci.2020.103826
关键词
LARK; Litopenaeus vannamei; Immune responses; Phagocytosis; Vibrio parahaemolyticus; White spot syndrome virus
资金
- National Key Research and Development Program of China [2018YFD0900505]
- National Natural Science Foundation of China [31772881, 31702371, 3192823]
- Natural Science Foundation of Guangdong Province, China [2017A030313190]
- China Agricultural Research System [CARS47]
- Fundamental Research Funds for the Central Universities of China [18lgpy57]
The study demonstrates that the LARK protein primarily regulates humoral immunity in shrimp, playing a positive role in antiviral and antibacterial responses. Silencing LARK increases susceptibility to specific pathogens, while also regulating expression of immune function proteins. This suggests a key role for LARK in immune response modulation.
The LARK proteins containing a C2HC-type zinc finger motif and two RNA recognition motifs are conserved across vertebrates and invertebrates. Previous studies have suggested that invertebrate LARKs and their mammalian counterparts, the RBM4 proteins, regulate gene expression by affecting RNA stability and post-transcriptional processing, participating in multiple life processes. In the current study, the LARK gene from Pacific white shrimp Litopenaeus vannamei was identified and functionally explored in the context of immunity. The LARK protein was mainly present in the nucleus of its expression vector-transfected S2 cells, and the LARK mRNA was detectable in all the tested shrimp tissues. Expression of LARK in gill was up-regulated by immune stimulation with various pathogens. In vivo experiments demonstrated that LARK played positive roles in both antiviral and antibacterial responses and silencing of LARK could make shrimp more susceptible to infection with Vibrio parahaemolyticus and white spot syndrome virus (WSSV). Although silencing of LARK did not affect the phagocytic activity of hemocytes, it regulated expression of many components of the NF-kappa B and JAK-STAT pathways and a series of immune function proteins. These suggested that LARK could be mainly involved in regulation of humoral immunity. The current study could help reveal the roles of LARK/RBM4 in immunity and further explore the regulatory mechanisms of shrimp immunity.
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