期刊
DEVELOPMENT
卷 147, 期 14, 页码 -出版社
COMPANY BIOLOGISTS LTD
DOI: 10.1242/dev.185777
关键词
Synovial joint development; Interzone; Single cell RNA-Seq; Articular cartilage; Ligament; Synovium; Chondrocyte; Meniscus; Gdf5
资金
- National Institutes of Health [R35GM124725, R25GM109441]
- Maryland StemCell Research Fund [2017-MSCRFF-3910, 90074850]
- Johns Hopkins University Medicine Discovery Fund
Synovial joint development begins with the formation of the interzone, a region of condensed mesenchymal cells at the site of the prospective joint. Recently, lineage-tracing strategies have revealed that Gdf5-lineage cells native to and from outside the interzone contribute to most, if not all, of the major joint components. However, there is limited knowledge of the specific transcriptional and signaling programs that regulate interzone formation and fate diversification of synovial joint constituents. To address this, we have performed single cell RNA-Seq analysis of 7329 synovial joint progenitor cells from the developing murine knee joint from E12.5 to E15.5. By using a combination of computational analytics, in situ hybridization and in vitro characterization of prospectively isolated populations, we have identified the transcriptional profiles of the major developmental paths for joint progenitors. Our freely available single cell transcriptional atlas will serve as a resource for the community to uncover transcriptional programs and cell interactions that regulate synovial joint development.
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