期刊
CYTOTECHNOLOGY
卷 72, 期 5, 页码 639-647出版社
SPRINGER
DOI: 10.1007/s10616-020-00402-x
关键词
Leucine aminopeptidase 3 (LAP3); Epithelial-mesenchymal transition (EMT); Vimentin; Metastasis
资金
- Project of Shandong Province Higher Educational Science and Technology Program [J17KA255]
- Foundation of Weifang Scientific Committee [2017YX064]
- University Students Science and Technology Innovation Fund Project [201910438027X, KX2019025]
Breast cancer is a common type of cancer in females. Our previous studies indicated that leucine aminopeptidase 3 (LAP3) promotes migration and invasion of breast cancer cells. Vimentin is a mesenchymal marker, and its upregulation represents the promotion of epithelial-mesenchymal transition. In this study, we found that LAP3 and vimentin were highly expressed in breast cancer tissues, and the overexpression of LAP3 in breast cancer cells promoted the expression of vimentin. Western blot analysis indicated that the overexpression of LAP3 upregulated the phosphorylation of Erk1/2. MEK inhibitor PD98059 downregulated the expression of vimentin, matrix metalloproteinase-2/9 (MMP-2/9), and fascin through the inhibition of Erk1/2 activity. We hypothesized that LAP3 promoted tumor migration and invasion by upregulating vimentin. The knockdown of vimentin resulted in the inhibited migration and invasion of MDA-MB-231 and MDA-MB-468 cells. The expression of MMP-2/9 and fascin could also be downregulated. In conclusion, vimentin might play an important role in the promotion of breast cancer metastasis by LAP3.
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