期刊
CURRENT OPINION IN CHEMICAL BIOLOGY
卷 57, 期 -, 页码 58-64出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.cbpa.2020.04.005
关键词
Semisynthetic fluorescent sensors; Fluorescent chemical-genetic hybrids; Fluorescence imaging; Biosensing
资金
- European Research Council [ERC-2016-CoG-724705 FLUOSWITCH]
Fluorescent biosensors are powerful tools for the detection of biochemical events inside cells with high spatiotemporal resolution. Biosensors based on fluorescent proteins often suffer from issues with photostability and brightness. On the other hand, hybrid, chemical-genetic systems present unique opportunities to combine the strengths of synthetic, organic chemistry with biological macromolecules to generate exquisitely tailored semisynthetic sensors.
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