期刊
CURRENT BIOLOGY
卷 30, 期 16, 页码 3116-+出版社
CELL PRESS
DOI: 10.1016/j.cub.2020.05.091
关键词
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资金
- National Institutes of Health [HD40182, GM105536]
- Fundacao para a Ciencia e a Tecnologia MDPhD Scholarship [PD/BD/113766/2015]
- Fulbright CONICYT Becas Chile [72170520]
- Columbia University Medical Center MD-PhD program
- Fundação para a Ciência e a Tecnologia [PD/BD/113766/2015] Funding Source: FCT
Vertebrate brain development depends on a complex program of cell proliferation and migration. Post-mitotic neuronal migration in the developing cerebral cortex involves Nesprin-2, which recruits cytoplasmic dynein, kinesin, and actin to the nuclear envelope (NE) in other cell types. However, the relative importance of these interactions in neurons has remained poorly understood. To address these issues, we performed in utero electroporation into the developing rat brain to interfere with Nesprin-2 function. We find that an similar to 100-kDa mini form of the similar to 800-kDa Nesprin-2 protein, which binds dynein and kinesin, is sufficient, remarkably, to support neuronal migration. In contrast to dynein's role in forward nuclear migration in these cells, we find that kinesin-1 inhibition accelerates neuronal migration, suggesting a novel role for the opposite-directed motor proteins in regulating migration velocity. In contrast to studies in fibroblasts, the actinbinding domain of Nesprin-2 was dispensable for neuronal migration. We find further that, surprisingly, the motor proteins interact with Nesprin-2 through the dynein/kinesin adaptor BicD2, both in neurons and in non-mitotic fibroblasts. Furthermore, mutation of the Nesprin-2 LEWD sequence, implicated in nuclear envelope kinesin recruitment in other systems, interferes with BicD2 binding. Although disruption of the Nesprin2/BicD2 interaction severely inhibited nuclear movement, centrosome advance proceeded unimpeded, supporting an independent mechanism for centrosome advance. Our data together implicate Nesprin-2 as a novel and fundamentally important form of BicD2 cargo and help explain BicD2's role in neuronal migration and human disease.
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