期刊
COLLOIDS AND SURFACES B-BIOINTERFACES
卷 191, 期 -, 页码 -出版社
ELSEVIER
DOI: 10.1016/j.colsurfb.2020.110999
关键词
ZnO nanorods (ZnO NRs); Whispering gallery modes (WGMs); Whispering gallery mode resonators; Quasi-whispering gallery modes (quasi-WGMs); Photoluminescence; Biosensors; Optical immunosensor; Optical resonators; Grapevine virus A-type (GVA) antigen/antibody-complex
资金
- Ukrainian-Lithuanian Research project Application of hybrid nanostructures which are based on TiO2 or ZnO and modified by biomolecules, in optoelectronic sensors Lithuanian Research Council [P-LU-18-53]
- Swedish Research Council (VR) Marie Sklodowska Curie International Grant [2015-00679]
- AForsk [14-517]
- Swedish Research Council [2015-00679] Funding Source: Swedish Research Council
In this research a whispering gallery mode (WGM) resonator based on vertically oriented ZnO nanorods, which were formed on silicon surface (silicon/ZnO-NRs), has been applied in the design of optical immunosensor that was dedicated for the determination of grapevine virus A-type (GVA) proteins. Vertically oriented ZnO-NRs were grown on silicon substrates by atmospheric pressure metal organic chemical vapor deposition (APMOCVD) and the silicon/ZnO-NRs structures formed were characterized by structural and optical methods. Optical characterization demonstrates that silicon/ZnO-NRs-based structures can act as 'whispering gallery mode' (WGM) resonator where quasi-whispering gallery modes (quasi-WGMs) are generated. These quasi-WGMs were experimentally observed in the visible and infrared ranges of the photoluminescence spectra. In order to design an immuno-sensing system the anti-GVA antibodies were immobilized on the surface of silicon/ZnO-NRs and in this way silicon/ZnO-NRs/anti-GVA structure was formed. The immobilization of anti-GVA antibodies and then the interaction of silicon/ZnO-NRs/anti-GVA structure with GVA proteins (GVA-antigens) resulted in an opposite shifts of the WGMs peaks in the visible range of the photoluminescence spectra observed as a defect-related photoluminescence emission of ZnO-NRs. Here designed silicon/ZnO-NRs/anti-GVA immuno-sensing structure demonstrates the sensitivity towards GVA-antigens in the concentration range of 1-200 ng/ml. Bioanalytical applicability of the silicon/ZnO-NRs-based structures in the WGMs registration mode is discussed.
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