期刊
COLLOIDS AND SURFACES B-BIOINTERFACES
卷 191, 期 -, 页码 -出版社
ELSEVIER
DOI: 10.1016/j.colsurfb.2020.110987
关键词
Carbon nanodots; Highly negative surface charge; Microbial viability
资金
- National Key R&D Program of China [2017YFF0108600, 2017YFC0211900, 2016YFF0103800]
- National Natural Science Foundation of China [81771982, 61535010, 8160071152, 21803075]
- Key Research Program of the Chinese Academy of Sciences [KFZD-SW-210]
- Natural Science Foundation of Jiangsu Province [BE2015601, BK20181236]
Assessment of microbial viability plays a key role in human health protection. Optical imaging based on fluorescent dyes is a simple and convenient way to assess microbial viability. However, it is still a challenge to obtain stable, nontoxic and low-cost dyes. Herein, we prepared a nitrogen and phosphorus co-doped carbon nanodots (N, P-CDs) via a one-step solvothermal method. The prepared CDs possess plenty of functional groups and exhibit high stability, good biocompatibility, excellent photoluminescent and low toxicity. Especially, the properties of high quantum yield (89.9%) and highly negative surface charge (-41.9 mV) make the prepared N, P-CDs ideal materials for microbial differentiation. Compared with commercial dyes, our CDs are more stable, cost less, which can rapidly distinguish dead microorganisms from living ones with higher specificity.
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