期刊
CLINICAL INFECTIOUS DISEASES
卷 73, 期 7, 页码 E1870-E1877出版社
OXFORD UNIV PRESS INC
DOI: 10.1093/cid/ciaa905
关键词
SARS-CoV-2; COVID-19; transmission; air contamination; surface contamination
资金
- NIHR Imperial Biomedical Research Centre
- NIHR Health Research Health Protection Research Unit (HPRU) in HCAI and Antimicrobial resistance (AMR)
- HPRU in Respiratory Infections at Imperial College
The study evaluated SARS-CoV-2 surface and air contamination in a London hospital during the COVID-19 pandemic. Viral RNA was detected on surfaces (52.3%) and in air samples (38.7%), but no virus was cultured. The high PCR cycle threshold indicated the virus was not culturable.
Background. We evaluated severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) surface and air contamination during the coronavirus disease 2019 (COVID-19) pandemic in London. Methods. Prospective, cross-sectional, observational study in a multisite London hospital. Air and surface samples were collected from 7 clinical areas occupied by patients with COVID-19 and a public area of the hospital. Three or four 1.0-m(3) air samples were collected in each area using an active air sampler. Surface samples were collected by swabbing items in the immediate vicinity of each air sample. SARS-CoV-2 was detected using reverse-transcription quantitative polymerase chain reaction (PCR) and viral culture; the limit of detection for culturing SARS-CoV-2 from surfaces was determined. Results. Viral RNA was detected on 114 of 218 (52.3%) surfaces and in 14 of 31 (38.7%) air samples, but no virus was cultured. Viral RNA was more likely to be found in areas immediately occupied by COVID-19 patients than in other areas (67 of 105 [63.8%] vs 29 of 64 [45.3%]; odds ratio, 0.5; 95% confidence interval, 0.2-0.9; P = .025, chi(2) test). The high PCR cycle threshold value for all samples (>30) indicated that the virus would not be culturable. Conclusions. Our findings of extensive viral RNA contamination of surfaces and air across a range of acute healthcare settings in the absence of cultured virus underlines the potential risk from environmental contamination in managing COVID-19 and the need for effective use of personal protective equipment, physical distancing, and hand/surface hygiene.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据