Highly selective enrichment of aflatoxin B1 from edible oil using polydopamine-modified magnetic nanomaterials

Aflatoxin B-1 (AFB(1)) is a highly toxic mycotoxin that enters the human body through the food chain and poses a serious threat to human health. In this paper, polydopamine (PDA)-coated Fe3O4 magnetic nanoparticles (Fe3O4@PDA MNPs) were prepared by the co-precipitation method to enrich aflatoxin from edible oil. Transmission electron microscopy (TEM), Fourier transform infrared spectroscopy, X-ray photoelectron spectroscopy, and vibrating sample magnetometer were used to characterize the Fe3O4@PDA MNPs. Using the obtained Fe3O4@PDA MNPs as an adsorbent, a simple method for enriching AFB(1) from samples by magnetic solid phase extraction (MSPE) combined with fluorescence rapid detection was developed. The effects of the ratio of Fe3O4 MNPs to PDA, adsorption dosage, sample volume, adsorption time, and elution time on enrichment of AFB(1) were investigated to determine the optimal experimental conditions. This method has good intraday and daytime precision.

Automated summary

In this study, a method for enriching AFB(1) using Fe3O4@PDA MNPs combined with fluorescence rapid detection was developed, showing good experimental conditions and precision.