期刊
CHINESE JOURNAL OF CHEMISTRY
卷 38, 期 12, 页码 1607-1611出版社
WILEY-V C H VERLAG GMBH
DOI: 10.1002/cjoc.202000284
关键词
Trioxacarcin; Membrane-boundO-acyltransferase; Catalytic mechanism; Trioxacarcin analog
资金
- NNSFC [21632007, 21621002]
- CAS [XDB20000000]
- CAS (K. C. Wong Education Foundation)
The Summary of main observation and conclusion Trioxacarcin A (TXN-A) is a polycyclic aromatic natural product with remarkable biological activity. TxnB11, a membrane-boundO-acetyltransferase involved in TXN-A biosynthesis that is difficult for protein manipulation, is biochemically characterized here for its acetylation function on C4-sugar. A proposed catalytic mechanism is supported by transmembrane helix analysis and site-directed mutagenesis, in which four conserved amino acid residues His35, Ser71, His100 and His317 are essential for enzyme activity. We tested the substrate tolerance of TxnB11 to acyl donorsin vitroand found that TxnB11 can also utilize propionyl-CoA and glycolyl-CoA, resulting in two new TXN analogs with anti-tumor activity. This work provides a first understanding of the catalytic mechanism of this unusual acyltransferase family and presents good candidates for creating structural diversity for natural products.
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