Site Specific Analysis of Anionic Lipid by Membrane Surface-enhanced Raman Spectroscopy with Different Sized Gold Nanoparticles

For highly sensitive analysis of anionic lipid membranes, we developed membrane surface enhanced Raman spectroscopy with different sized gold nanoparticles (AuNPs). The employed AuNPs did not disturb and retained native lipid membrane properties such as fluidity and polarity, and enhanced Raman signals of anionic lipid membrane. Furthermore, the enhanced part of lipid membrane was modulated by different size AuNPs. Thus, our method enables site specific analysis of anionic lipid membrane in high sensitive manner keeping native lipid membrane properties.