期刊
CHEMBIOCHEM
卷 21, 期 23, 页码 3323-3328出版社
WILEY-V C H VERLAG GMBH
DOI: 10.1002/cbic.202000344
关键词
DNA; liquid-liquid phase separation; membraneless cell organelles; microdroplets; synthetic biology
资金
- JSPS KAKENHI [JP15H02121, JP18J12947, JP18H04976, JP19K06540, JP20H01877]
- MEXT KAKENHI [JP25103012, JP24104004]
- Japan Society for Promotion of Science
Recently, liquid-liquid phase separation (LLPS) has attracted considerable attention among researchers in the life sciences as a plausible mechanism for the generation of microstructures inside cells. LLPS occurs through multiple nonspecific interactions and does not always require a lock-and-key interaction with a binary macromolecular solution. The remarkable features of LLPS include the non-uniform localization and concentration of solutes, resulting in the ability to isolate certain chemical systems and thereby parallelize multiple chemical reactions within the limited space of a living cell. We report that, by using the macromolecules, poly(ethylene glycol) (PEG) and dextran, that exhibit LLPS in an aqueous solution, cell-sized liposomes are spontaneously formed therein in the presence of phospholipids. In this system, LLPS is generated through the depletion effect of macromolecules. The results showed that cell-like microdroplets entrapping DNA wrapped by a phospholipid layer emerge in a self-organized manner.
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