期刊
CERAMICS INTERNATIONAL
卷 46, 期 11, 页码 17651-17658出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.ceramint.2020.04.067
关键词
Surface modification; Picosecond laser ablation; Aluminum oxide structures; Fish-scale structures; Cell culture
资金
- Ministry of Science and Technology (MOST) of Taiwan, Republic of China [MOST 107-2221-E003-006-MY2, MOST 108-2622-E-003-001-CC3]
- National Defense Medical Center [MAB-108-077]
The surface modification of a layer substrate has received attention for many biomedical applications (e.g., tissue engineering and biosensing). Under an in situ control, we proposed a strategy for surface modification of hard and brittle ceramics with ultrafast laser pulses for cell culture. A type of ultrafast laser based on the picosecond pulse technique was employed to ablate the surface of a pristine aluminum oxide (Al2O3) substrate. The surface of the formed reproducible micro-nanostructures indicated the fish-scale structures, which was analyzed under different laser ablation conditions. Subsequently, the SV40 T-antigen human embryonic kidney 293 (HEK293T) cells were cultured on the laser-ablated Al2O3 substrates. These microstructures and nanostructures can enhance cell proliferation response. The surface effect played a crucial role, by which the surface roughness (R-a) and contact angle (CA) of water droplets on the ablated Al2O3 substrate increased with the increasing laser fluence. The formed fish-scale structures were formed by the parameters of laser ablation where the structural characteristics of the Al2O3 substrate can be remained in the ultrafast laser process. The presented process provides a simple support for developing ablated structures on the Al2O3 substrate for cell culture.
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