期刊
CELLULAR SIGNALLING
卷 70, 期 -, 页码 -出版社
ELSEVIER SCIENCE INC
DOI: 10.1016/j.cellsig.2020.109577
关键词
Genome editing; CRISPR; Cas9; Off-target
类别
资金
- Ministry of Science and Technology of China [2018YFC1004603]
- National Natural Science Foundation of China [31872831]
- Science and Technology Commission of Shanghai Municipality [19JC1413800]
- Shanghai Pujiang program [18PJ1407900]
- Shanghai Shuguang Program [19SG55]
- ShanghaiTech University Startup grant
As a revolutionary gene editing tool based on the adaptive immune defense mechanism of bacteria and archaea against exogenous DNA invasion, CRISPR/Cas system shows many remarkable characteristics over ZFNs and TALENs. However, off-target effect remains as one of the major imperfection of CRISPR/Cas system, hindering its further application in translational research. In this review, we highlight major breakthrough cross the development/application of this powerful toolkit, and summarize feasible methods for detecting potential off-target effects during genetic manipulation. We hope this review will assist scientists for accurate genomic editing in their future research.
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