期刊
CELLULAR MICROBIOLOGY
卷 22, 期 10, 页码 -出版社
WILEY
DOI: 10.1111/cmi.13246
关键词
Acanthamoeba; amoebae; bacterial evolution; Coxiella; Dictyostelium; effector protein; endoplasmic reticulum; endosome; host-pathogen interaction; Legionella; macrophage; microtubule; pathogen vacuole; phosphoinositide lipid; small GTPase; type IV secretion
资金
- Agence Nationale de la Recherche [ANR-10-LABX-62-IBEID]
- Fondation pour la Recherche Medicale [EQU201903007847]
- Novartis Stiftung fur Medizinisch-Biologische Forschung
- OPO Foundation
- Schweizerischer Nationalfonds zur Forderung der Wissenschaftlichen Forschung [31003A_153200, 31003A_175557]
Intracellular bacterial pathogens harbour genes, the closest homologues of which are found in eukaryotes. Regulator of chromosome condensation 1 (RCC1) repeat proteins are phylogenetically widespread and implicated in protein-protein interactions, such as the activation of the small GTPase Ran by its cognate guanine nucleotide exchange factor, RCC1.Legionella pneumophilaandCoxiella burnetii, the causative agents of Legionnaires' disease and Q fever, respectively, harbour RCC1 repeat coding genes.Legionella pneumophilasecretes the RCC1 repeat 'effector' proteins LegG1, PpgA and PieG into eukaryotic host cells, where they promote the activation of the pleiotropic small GTPase Ran, microtubule stabilisation, pathogen vacuole motility and intracellular bacterial growth as well as host cell migration. The RCC1 repeat effectors localise to the pathogen vacuole or the host plasma membrane and target distinct components of the Ran GTPase cycle, including Ran modulators and the small GTPase itself.Coxiella burnetiitranslocates the RCC1 repeat effector NopA into host cells, where the protein localises to nucleoli. NopA binds to Ran GTPase and promotes the nuclear accumulation of Ran(GTP), thus pertubing the import of the transcription factor NF-kappa B and innate immune signalling. Hence, divergent evolution of bacterial RCC1 repeat effectors defines the range of Ran GTPase cycle targets and likely allows fine-tuning of Ran GTPase activation by the pathogens at different cellular sites.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据