期刊
CELL PROLIFERATION
卷 53, 期 9, 页码 -出版社
WILEY
DOI: 10.1111/cpr.12870
关键词
Differentiation; GWAS; NGS; NMW; Proliferation
类别
资金
- Cattle Breeding Innovative Research Team [ASTIPIAS03, ZDXT2018006]
Objectives Genome-wide association studies (GWAS) represent a powerful approach to detecting candidate genes for economically important traits in livestock. Our aim was to identify promising candidate muscle development genes that affect net meat weight (NMW) and validate these candidate genes in cattle. Materials and methods Using a next-generation sequencing (NGS) dataset, we applied similar to 12 million imputed single nucleotide polymorphisms (SNPs) from 1,252 Simmental cattle to detect genes influencing net meat yield by way of a linear mixed model method. Haplotype and linkage disequilibrium (LD) blocks were employed to augment support for identified genes. To investigate the role ofMTPNin bovine muscle development, we isolated myoblasts from the longissimus dorsi of a bovine foetus and treated the cells during proliferation, differentiation and hypertrophy. Results We identified one SNP(rs100670823) that exceeded our stringent significance threshold (P = 8.58 x 10(-8)) for a putative NMW-related quantitative trait locus (QTL). We identified a promising candidate gene,myotrophin(MTPN), in the region around this SNPMyotrophinhad a stimulatory effect on six muscle-related markers that regulate differentiation and myoblast fusion. During hypertrophy,myotrophinpromoted myotube hypertrophy and increased myotube diameters. Cell viability assay and flow cytometry showed thatmyotrophininhibited myoblast proliferation. Conclusions The present experiments showed thatmyotrophinincreases differentiation and hypertrophy of skeletal muscle cells, while inhibiting their proliferation. Our examination of GWAS results with in vitro biological studies provides new information regarding the potential application ofmyotrophinto increase meat yields in cattle and helpful information for further studies.
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