期刊
CARBOHYDRATE POLYMERS
卷 249, 期 -, 页码 -出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.carbpol.2020.116894
关键词
Lycii fructus; Polysaccharide; Purification; Structure characterization; Neurotrophic activity; Neuroprotective activity
资金
- National Natural Science Foundation of China [81773837, 81960711, 81703396]
- key project of Jiangsu Collaborative Innovation Center of Chinese Medicinal Resources Industrialization [012092002001-10]
- Priority Academic Program Development [PAPD]
- Top-notch Academic Program Project [TAPP]
- Flagship Major Development of Jiangsu Higher Education Institutions [ZY2015A070]
Regeneration of neurites network constitutes a neurotrophic and therapeutic strategy for Parkinson's disease (PD). Increasing evidence is supporting the potential application of natural polysaccharides in prevention or treatment of PD. In this study, an acidic heteropolysaccharide LFP-1 was isolated from Lycii fructus, and purified by ion-exchange and gel filtration chromatography. Structural features of LFP-1 were analyzed with molecular weight (MW) distribution, monosaccharide composition, methylation and nuclear magnetic resonance (NMR) spectra. LFP-1 was a complicated structured polysaccharide with an average MW of 1.78 x 10(4) Da and composed of highly branched arabinogalactans, homogalacturonan and rhamnogalacturonan moieties. LFP-1 promoted neuronal differentiation and neurite outgrowth in vitro in PC12 cell models. Furthermore, LFP-1 had a signifi-cantly protective effect against 1-methyl-4-phenylpyridiniumion (MPP+)-induced neurotoxicity in PD model PC12 cells. These observations unambiguously indicated the neurotrophic and neuroprotective activities of LFP-1, which may be developed for prevention or treatment of neurodegeneration in PD.
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