期刊
BMC VETERINARY RESEARCH
卷 16, 期 1, 页码 -出版社
BMC
DOI: 10.1186/s12917-020-02467-4
关键词
Feline morbillivirus; Fusion; Hemagglutinin; Phosphoprotein; Selective pressure analysis; Urine
资金
- 100th Anniversary Chulalongkorn University Fund for Doctoral Scholarship
- Ratchadaphisek Somphot Fund for Postdoctoral Fellowship, Chulalongkorn University
- Thailand Research Fund [RSA6180034]
- Veterinary Science Research Fund, Chulalongkorn University [RES_61_364_31_037]
- Grant for Joint Funding of External Research Project
- Ratchadaphisek Somphot Endowment Fund
- Overseas Presentations of Graduate Level Academic Thesis Scholarship
- 90th Anniversary of Chulalongkorn University Fund (Ratchadaphisek Somphot Endowment Fund)
Background Feline morbillivirus (FeMV) has been discovered in domestic cats associated with tubulointerstitial nephritis, but FeMV is also detected in healthy cats. This research aimed to identify and characterize the FeMV strains detected in a Thai cat population. Results Two-hundred and ninety-two samples (131 urine and 161 blood) derived from 261 cats (61 sheltered and 200 household cats) were included for investigating the FeMV prevalence using real-time reverse transcription PCR. The overall prevalence of FeMV detection was 11.9% (31/261) among both samples, which accounted for 14.5% (19/131) and 7.5% (12/161) of the urine and blood samples, respectively. Among the FeMV-PCR positive cats, the FeMV-detected prevalence was insignificantly associated with healthy cats (58.1%; 18/31) or urologic cats (41.9%; 13/31). Full-length genome analysis of these FeMV-Thai strains revealed that their genomes clustered together in the FeMV-1A clade with up to 98.5% nucleotide identity. Selective pressure analysis showed that overall FeMV-1 has undergone negative selection, while positive selection sites were more frequently observed in the phosphoprotein gene. Conclusions The detected FeMV infections in the Thai cat population were not correlated with urologic disorders, although the virus was more detectable in urine samples. The genetic patterns among the FeMV-1 Thai strains were more consistent. A large-scale study of FeMV in Thai cat samples is needed for further elucidation.
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