期刊
BIOTECHNOLOGY AND BIOENGINEERING
卷 117, 期 11, 页码 3499-3507出版社
WILEY
DOI: 10.1002/bit.27511
关键词
Escherichia coli; membrane extension; metabolic engineering; mevalonate pathway; squalene
资金
- National Key R&D Program of China [2019YFA0905200]
- National Natural Science Foundation of China [21878198]
- Next-Generation BioGreen 21 Program (SSAC) [PJ01326501]
- RDA
- National Research Foundation of Korea - MSIP, Korea [NRF-2016R1A2B2010678]
- Suzhou Science and Technology Bureau [SNG201916]
- Priority Academic Program Development of Jiangsu Higher Education Institutions (PAPD)
- Rural Development Administration (RDA), Republic of Korea [PJ013265012020] Funding Source: Korea Institute of Science & Technology Information (KISTI), National Science & Technology Information Service (NTIS)
Squalene is a lipophilic and non-volatile triterpene with many industrial applications for food, pharmaceuticals, and cosmetics. Metabolic engineering focused on optimization of the production pathway suffer from little success in improving titers because of a limited space of the cell membrane accommodating the lipophilic product. Extension of cell membrane would be a promising approach to overcome the storage limitation for successful production of squalene. In this study,Escherichia coliwas engineered for squalene production by overexpression of some membrane proteins. The highest production of 612 mg/L was observed in the engineeredE.coliwith overexpression of Tsr, a serine chemoreceptor protein, which induced invagination of inner membrane to form multilayered structure. It was also observed an increase in unsaturated fatty acid in membrane lipids composition, suggesting cellular response to maintain membrane fluidity against squalene accumulation in the engineered strain. This study potentiates the capability ofE. colifor squalene production and provides an effective strategy for the enhanced production of such compounds.
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