期刊
BIOSENSORS & BIOELECTRONICS
卷 158, 期 -, 页码 -出版社
ELSEVIER ADVANCED TECHNOLOGY
DOI: 10.1016/j.bios.2020.112176
关键词
Quartz crystal resonator (QCR); Quartz crystal microbalance (QCM); Fixed frequency drive; Nanomolecular imprinted polymer (nano-MIP); Acyl-homoserine lactone (AHL)
类别
资金
- EU [FP7-JTI 115153, FP7-NMP 604244]
- EPSRC Bridging the Gap in Antimicrobial Resistance grant [EP/M027341/1]
- Wolfson School
- EPSRC [EP/M027341/1] Funding Source: UKRI
Small molecule detection is of wide interest in clinical and industrial applications. However, its accessibility is still limited as miniaturisation and system integration is challenged in reliability, costs and complexity. Here we combined a 14.3 MHz quartz crystal resonator (QCR), actuated and analysed using a fixed frequency drive (FFD) method, with a nanomolecular imprinted polymer for label-free, realtime detection of N-hexanoyl-L-homoserine lactone (199 Da), a gram-negative bacterial infection biomarker. The lowest concentration detected (1 mu M) without any optimisation was comparable with that of a BIAcore SPR system, an expensive laboratory gold standard, with significant enhancement in sensitivity and specificity beyond the state-of-the-art QCR. The analytical formula-based FFD method can potentially allow a multiplexed QCR-on-chip technology, bringing a paradigm shift in speed, accessibility and affordability of small molecule detection.
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