期刊
BIOSENSORS & BIOELECTRONICS
卷 157, 期 -, 页码 -出版社
ELSEVIER ADVANCED TECHNOLOGY
DOI: 10.1016/j.bios.2020.112163
关键词
Bacterial cellulose; High-density phage immobilization; Electrochemical biosensor; Sensitivity and selectivity; Live/dead cell discrimination; Staphylococcus aureus detection
类别
资金
- National Key Research and Development Program of China [2017YFC1104402]
- China Postdoctoral Science Foundation [2016M602291]
- initial Research fund from the Chinese Scholarship Council (CSC)
- 3551 Project, Optics Valley of China
The routinely used enzymes, antibodies, and nucleic acids-based biosensors for detection of Staphylococcus aureus are often overwhelmed by limited selectivity, sensitivity, high cost, and inability to discriminate between live/dead cells. This necessitates the development of an ultra-sensitive, stable, and selective electrochemical biosensor capable of discriminating live S. aureus in a mixture of live/dead cells in food samples. The current study reports the development of an electrochemical biosensor through the immobilization of bacteriophage in surface-modified bacterial cellulose (BC) matrix. BC being highly porous and fibrous, offers a high surface area for the impregnation of carboxylated multiwalled carbon nanotubes (c-MWCNTs) and allows high-density phage immobilization. Surface modification of BC/c-MWCNTs with polyethyleneimine (PEI) provides a positive charge that facilitates oriented phage immobilization. FE-SEM and FT-IR analyses confirmed the development of BC/c-MWCNTs-PEI-phage bio-interface. Confocal microscopy analysis showed 11.7 +/- 1.2 phage particles.mu m(-2) immobilized in the BC matrix and showed anti-staphylococcal activity by producing clear lytic zone and reduced bacterial growth. Differential pulse voltammetry (DPV) analysis detected 3 CFU.mL(-1) and 5 CFU.mL(-1) of S. aureus in phosphate buffer saline (PBS) and milk, respectively, within 30 min at neutral pH and showed stability over 6-weeks at 4 degrees C. The biosensor showed high specificity for S. aureus, both in pure and mixed cultures of non-host bacteria, and effectively discriminated live S. aureus in a mixture of live/dead cells. The developed biosensor represents a simple, sensitive, specific, and accurate tool for early detection of S. aureus in food samples.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据