期刊
BIOSCIENCE REPORTS
卷 40, 期 -, 页码 -出版社
PORTLAND PRESS LTD
DOI: 10.1042/BSR20193169
关键词
-
资金
- Study on the proliferation mechanism and diagnostic value of serum lncRNA SRPA3 in elderly non-small cell lung cancer [2019SF-185]
- Study on the diagnostic value of differential lncRNAs expression profile in pleural effusion in elderly patients [201805093YX1SF27(9)]
- Program for Research Projects of the Shaanxi Provincial Health Department [2016D036, 2018D019]
- Shaanxi Provincial Traditional Chinese Medicine Administration Chinese Medicine Research Project [JCPT039]
- Research for Projects of the Xi'an technology office program [2016047SF/YX03(4)]
- Key Projects of Social Development Research Programs of Shaanxi Province [2017SF-199]
- Natural Science Basic Research Program of Shaanxi Province [2018JM-7044]
- Study on the role and mechanism of MUC in chemotherapy resistance of LUAD [201805093YX1SF27(10)]
Six-transmembrane epithelial antigen of prostate-1 (STEAP1) is a relatively newly identified gene target from prostate cancer, breast cancer, and gastric cancer. However, functions of STEAP1 in lung adenocarcinoma (LUAD) are still unknown. In the present study, we explored the molecular and cellular mechanisms of STEAP1 in LUAD. Western blot and Q-PCR were conducted to detect the protein and mRNA expressions respectively. The cell proliferation was tested by CCK8 assay. The effects of STEAP1 on the metastasis and epithelial-mesenchymal transition (EMT) of LUAD were evaluated by EdU assay, wound healing assay, and transwell migratory assay. H1650, H358, HCC827, H1299, H23, A549, H1693 were selected as human LUAD cell lines in the study. Results have shown that STEAP1 expression was up-regulated in LUAD cells compared with normal lung epithelial cells. Knockdowning of STEAP1 suppressed the proliferation, migration, and invasion of LUAD epithelial cells. Importantly, after comparing the proliferation, migration, and invasion of LUAD to the corresponding control groups treated in STAT3 inhibitor ADZ1480, we found that STEAP1 regulates EMT via Janus kinase 2/signal transducer and activator of transcription 3 (JAK2/STAT3) signaling pathway. In conclusion, STEAP1 can serve as a therapeutic target, and it may have important clinical implications for LUAD treatment.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据