期刊
BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY
卷 84, 期 9, 页码 1948-1957出版社
TAYLOR & FRANCIS LTD
DOI: 10.1080/09168451.2020.1780112
关键词
chemotaxis to amino acids; methyl-accepting chemotaxis protein; Pseudomonas protegens CHAO; plant-growth-promoting rhizobacteria
类别
资金
- Japan Society for the Promotion of Science [15H04478]
- Grants-in-Aid for Scientific Research [15H04478] Funding Source: KAKEN
Pseudomonas protegens: CHAO, known as plant-growth-promoting rhizobacterium, showed positive chemotactic responses toward proteinaceous L-amino acids. Genomic analysis revealed that P. protegens CHAO possesses four putative chemoreceptors for amino acids (designated CtaA, CtaB, CtaC, and CtaD, respectively). Pseudomonas aeruginosa PCT2, a mutant defective in chemotaxis to amino acids, harboring a plasmid containing each of ctaA, ctaB, ctaC, and ctaD showed chemotactic responses to 20, 4, 4, and 11 types of amino acids, respectively. To enhance chemotaxis toward amino acids, we introduced the plasmids containing ctaA, ctaB, ctaC, or ctaD into P. protegens CHAO. By overexpression of the genes, we succeeded in enhancing chemotaxis toward more than half of the tested ligands. However, unexpectedly, the P. protegens CHAO transformants showed unchanged or decreased responses to some amino acids when compared to wild-type CHAO. We speculate that alternation of expression of a chemoreceptor may affect the abundance of other chemoreceptors.
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