期刊
BIORESOURCE TECHNOLOGY
卷 309, 期 -, 页码 -出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.biortech.2020.123362
关键词
Porphyridium purpureum; C/N ratio; Phycoerythrin; Exopolysaccharides; Fatty acids
资金
- National Key Research and Development Project of China [2019YFA0906300]
- Natural Science Foundation of Shandong Province [ZR2019ZD17]
- National Natural Science Foundation of China [31872608]
- Natural Science Foundation of Shanghai [18ZR1410100, 17ZR1406700]
- Shanghai Pujiang Program [18PJD008]
- State Key Laboratory of Bioreactor Engineering
An efficient biomass and high-value bioactive substances production strategy was developed for unicellular microalgae Porphyridium purpureum. We studied the optimal culture method and metabolites accumulation under different C/N conditions, and further proposed methods to increase the yield under high C/N ratio. The highest biomass reached 16.24 g/L with ASW medium by mixotrophy. High C/N ratio and mediate C/N can significantly promote the synthesis and secretion of polysaccharides, as well as the accumulation of omega-6 PUFAs; however, inhibit the growth, resulting in lower yield. With the significant increase of C/N ratio, protein degradation was accelerated, providing sufficient nitrogen source for efficient accumulation of carbohydrates (1.66 g/L EPS) and PUFAs (231.24 mg/L ARA). Finally, we reduced the growth inhibition, shortened the culture cycle, and doubled the final biomass to 9.34 g/L under nitrogen deficiency condition. Our exploitation of a cost-effective and feasible culture method for red algae is particularly significant.
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