Water activity and biomass estimation using digital image processing in solid-state fermentation

Water activity in biotechnological applications plays a vital role. Estimation and precise control of water activity will enhance the product. The objective here is to use digital image processing technique in MATLAB for the quantification of the biomass and water condensate on a horizontal surface. A model has been developed with the data produced experimentally in this study in abiotic conditions. Also, a comparative study has been given for the estimation of the water condensate produced. It was observed that at a particular threshold value the error rate is less than 30%. The result of experimental study was good, and it showed a linear relation with proposed model. Also, the similar experimental estimation was done in biotic conditions, and results are showing good agreement between experimental observation and the model developed. Further, this method could be used in solid state fermentation process for water activity estimation and low-cost biomass estimation.