期刊
BIORESOURCE TECHNOLOGY
卷 305, 期 -, 页码 -出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.biortech.2020.123067
关键词
Cell factory; Thermococcus onnurineus NA1; Coproduction; Hydrogen; Thermophilic enzyme
资金
- Korea Institute of Ocean Science and Technology (KIOST) in-house program [PE99822]
- Ministry of Oceans and Fisheries in the Republic of Korea
- Korea Institute of Marine Science & Technology Promotion (KIMST) [PE99822] Funding Source: Korea Institute of Science & Technology Information (KISTI), National Science & Technology Information Service (NTIS)
To develop a thermophilic cell factory system that uses CO gas, we attempted to engineer a hyperthermophilic carboxydotrophic hydrogenic archaeon Thermococcus onnurineus NA1 to be capable of producing thermophilic enzymes along with hydrogen (H-2). The mutant strains 156T-AM and 156T-POL were constructed to have another copy of a gene encoding alpha-amylase or DNA polymerase, respectively, and exhibited growth rates and H-2 production rates distinct from those of the parental strain, 156T, in gas fermentation using 100% CO or coal-gasified syngas. Purified alpha-amylase displayed starch-hydrolyzing activity, and whole-cell extracts of 156T-AM showed saccharifying activity for potato peel waste. PCR amplification was used to demonstrate that purified DNA polymerase was free from bacterial DNA contamination, in contrast to commercial bacteria-made enzymes. This study demonstrated that this archaeal strain could coproduce enzymes and H-2 using CO-containing gas, providing a basis for cell factories to upcycle industrial waste gas.
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