期刊
BIOORGANIC CHEMISTRY
卷 101, 期 -, 页码 -出版社
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.bioorg.2020.104047
关键词
Phytosterols; Bovine serum albumin; Aggregations; Glycations; Multispectral; Molecular docking modeling
资金
- Hubei Provincial Natural Science Foundation for Innovative Group [2019CFA011]
- Chinese Scholarships Council [2017SLJ023757]
Discovering small molecules with protein-disaggregation effects is recently needed. For the first time, we in-tensely studied the anti-amyloidogenic effects of 3 structurally different phytosterols (PS), namely stigmasterol, beta-sitosterol, and gamma-oryzanol, on bovine serum albumin (BSA) under aggregations-promoting conditions using multispectral, microstructure, and molecular docking methods. Results found that PS dose-and structure- de-pendently inhibited BSA-aggregations under the glycation conditions through separating BSA-peak size, quenching Tryptophan-intensity, altering BSA-hydrophobicity, and microstructural declining the aggregates of glycated-BSA. Throughout the underlying mechanism beyond its disaggregation effects, PS reformed cross-beta-sheet structure, SDS-PAGE-bands, and XRD-peaks of glycated-BSA aggregates. Most importantly, PS were found to bind with some lysyl and arginine glycation sites of BSA, specifically Lys114, Lys116, Lys136, Lys431, Arg427, and Arg185, via Hydrogen-bonding with their -OH-groups and pi-pi interactions of their steroid core. Taken together, the current results unleash that PS could restrict BSA-aggregations under the glycation conditions and their subsequent changes, which can assist in the design of reasonable therapeutics.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据