期刊
BIOMEDICAL ENGINEERING ONLINE
卷 19, 期 1, 页码 -出版社
BMC
DOI: 10.1186/s12938-020-00797-w
关键词
Sonodynamic therapy; Sinoporphyrin sodium; Colorectal cancer
资金
- National Natural Science Foundation of China [81672904, 61427806]
- National Key Research and Development Program of China [2015BAI01B02]
- Project of Department of Education of Guangdong Province [2016KTSCX123]
- Postgraduate Innovation Development Fund of Shenzhen University [315-0000470204]
- Medical Young Scientists Program of Shenzhen University [1]
Background Colorectal cancer is the third leading cause of cancer-related deaths worldwide. Sonodynamic therapy (SDT) is an emerging cancer therapy, and in contrast to photodynamic therapy, could non-invasively reach deep-seated tissues and locally activates a sonosensitizer preferentially accumulated in the tumor area to produce cytotoxicity effects. In comparison with traditional treatments, SDT may serve as an alternative strategy for human colon cancer treatment. Here, we investigated the sonodynamic effect using sinoporphyrin sodium (DVDMS) as a novel sonosensitizer on human colon cancer cells in vitro. Results The absorption spectra of DVDMS revealed maximum absorption at 363 nm wavelength and emission peak at 635 nm. Confocal microscopy images revealed the DVDMS was primarily localized in the cytoplasm, while no evident signal was detected within the nuclei. Flow cytometry analysis showed rapid intracellular uptake of DVDMS by two types of human colon cancer cells (HCT116 and RKO). Cell viability of HCT116 was tolerant with the concentration of DVDMS up to 20 mu g/mL, while the case of RKO was 5 mu g/mL. In comparison with the control group, the SDT-treated groups of these two types of human colon cancer cells showed significant increase in cellular apoptosis and necrosis ratio. Increased intracellular reactive oxygen species (ROS) production was detected, indicating the involvement of ROS in mediating SDT effects. Conclusion DVDMS results an effective sonosensitizer for the ultrasound-mediated cancer cell killing, and its anticancer effect seems to rely on its ability to produce ROS under ultrasound exposure.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据