期刊
BIOMATERIALS
卷 242, 期 -, 页码 -出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.biomaterials.2020.119924
关键词
Dual organelles; Multiple tasks; Two-color emissions; AIEgen; Evaluating cell viability
资金
- National Natural Science Foundation of China [21788102]
- University Grants Committee of Hong Kong [AoE/P-03/08, AoE/P-02/12]
- Research Grants Council of Hong Kong [16305015, A-HKUST 605/16, C6009-17G]
- Innovation and Technology Commission [ITC-CNERC14SC01]
- Science and Technology Plan of Shenzhen [JCYJ2017081 8113851132, JCYJ20170818113840164, JCYJ20170818113530705, JCYJ20160229205601482, JCYJ20180507183832744]
Fully understanding the complicated interplays among various chemical species and organelles is greatly important to unravel the mystery of life. However, fluorescent probes capable of visualizing multiple targets discriminatively are severely deficient, which extremely limit the investigation on intracellular interplays among various species. Towards this end and in consideration of the unique advantages of aggregation-induced emission luminogens (AIEgens), here we rationally designed and presented a single AIEgen, named TVQE, bearing lipophilic, cationic and hydrolyzable moieties, and this AIEgen was capable of illuminating mitochondria and lipid droplets with red and blue emission, respectively. In addition, TVQE was successfully used for evaluating cell viability due to its distinct two-color emission changes tuned by esterase-mediated hydrolysis. Of particular importance is that TVQE can selectively differentiate live, early apoptotic, late apoptotic, and dead cells by confocal microscopy and quantify cell viability statistically by flow cytometry.
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