4.4 Article

Potent Inhibition of Mandelate Racemase by Boronic Acids: Boron as a Mimic of a Carbon Acid Center

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BIOCHEMISTRY
卷 59, 期 33, 页码 3026-3037

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AMER CHEMICAL SOC
DOI: 10.1021/acs.biochem.0c00478

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  1. Natural Sciences and Engineering Research Council (NSERC) of Canada [RGPIN-2016-05083, RGPIN-2016-05795]

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Boronic acids have been successfully employed as inhibitors of hydrolytic enzymes. Typically, an enzymatic nucleophile catalyzing hydrolysis adds to the electrophilic boron atom forming a tetrahedral species that mimics the intermediate(s)/transition state(s) for the hydrolysis reaction. We show that para-substituted phenylboronic acids (PBAs) are potent competitive inhibitors of mandelate racemase (MR), an enzyme that catalyzes a 1,1-proton transfer rather than a hydrolysis reaction. The K-i value for PBA was 1.8 +/- 0.1 nM, and p-Cl-PBA exhibited the most potent inhibition (K-i = 81 +/- 4 mu M), exceeding the binding affinity of the substrate by similar to 4 orders of magnitude. Isothermal titration calorimetric studies with the wild-type, K166M, and H297N MR variants indicated that, of the two Bronsted acid-base catalysts Lys 166 and His 297, the former made the greater contribution to inhibitor binding. The X-ray crystal structure of the MR.PBA complex revealed the presence of multiple H-bonds between the boronic acid hydroxyl groups and the side chains of active site residues, as well as formation of a His 297 N-epsilon 2-B dative bond. The dramatic upfield change in chemical shift of 27.2 ppm in the solution-phase B-11 nuclear magnetic resonance spectrum accompanying binding of PBA by MR was consistent with an sp(3)-hybridized boron, which was also supported by density-functional theory calculations. These unprecedented findings suggest that, beyond substituting boron at carbon centers participating in hydrolysis reactions, substitution of boron at the acidic carbon center of a substrate furnishes a new approach for generating inhibitors of enzymes catalyzing the deprotonation of carbon acid substrates.

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