期刊
ARCHIVES OF VIROLOGY
卷 165, 期 10, 页码 2373-2377出版社
SPRINGER WIEN
DOI: 10.1007/s00705-020-04737-w
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- Louisiana State University, School of Veterinary Medicine start-up fund [PG 002165]
- NIH-CEIRS, and NBAF Transition Funds from the State of Kansas
- NIAID Centers of Excellence for Influenza Research and Surveillance [HHSN 272201400006C]
In situhybridization (ISH) and immunohistochemistry (IHC) are essential tools to characterize SARS-CoV-2 infection and tropism in naturally and experimentally infected animals and also for diagnostic purposes. Here, we describe three RNAscope(R)-based ISH assays targeting the ORF1ab, spike, and nucleocapsid genes and IHC assays targeting the spike and nucleocapsid proteins of SARS-CoV-2.
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