期刊
APPLIED SPECTROSCOPY REVIEWS
卷 56, 期 5, 页码 380-422出版社
TAYLOR & FRANCIS INC
DOI: 10.1080/05704928.2020.1796698
关键词
Raman spectroscopy; SERS; bacteria detection; bacteria quantification; SERS substrates
SERS technique has been widely used in bacteria analysis, offering advantages in terms of analysis time, sensitivity, and sample preparation compared to traditional bacteria culture methods. Over the past 20 years, various SERS methods have been developed for bacteria analysis, with a comprehensive study of related technical issues.
Surface Enhanced Raman spectroscopy (SERS) technique was developed to provide a high enhancement of Raman scattering from molecules adsorbed on nanostructured noble metal surfaces. This technique has gained tremendous interest in the past few years for the detection, identification and quantification of bacteria. Indeed, compared to the standard methods based on bacteria culture, SERS has many advantages in particular in terms of analysis time, sensitivity and sample preparation. The aim of this review is to provide an overview and a discussion of the SERS methods developed for bacteria analysis over the last 20 years. To well assess the technical issues of such developments, this literature review is presented after having described the physical and chemical phenomena involved in the Raman scattering enhancement as well as the different types of noble metal substrates that can be used.
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