期刊
CHINESE MEDICAL JOURNAL
卷 129, 期 14, 页码 1711-1718出版社
MEDKNOW PUBLICATIONS & MEDIA PVT LTD
DOI: 10.4103/0366-6999.185862
关键词
Candida albicans; Infection; Inflammation; Intestinal Mucosa Barrier; Ischemia-reperfusion Injury
资金
- Research Foundation of Shanghai Minhang District Municipal Commission of Health and Family Planning [2013MW12]
- Research Foundation of Shanghai Municipal Commission of Health and Family Planning [201540136]
Background: Inflammation is supposed to play a key role in the pathophysiological processes of intestinal ischemia-reperfusion injury (IIRI), and Candida albicans in human gut commonly elevates inflammatory cytokines in intestinal mucosa. This study aimed to explore the effect of C. albicans on IIRI. Methods: Fifty female Wistar rats were divided into five groups according to the status of C albicans infection and IIRI operation: group blank and sham; group blank and IIRI; group cefoperazone plus IIRI; group C albicans plus cefoperazone and IIRI (CCI); and group C. albicans plus cefoperazone and sham. The levels of inflammatory factors tumor necrosis factor (TNF)-alpha, interleukin (IL)-6, IL-beta, and diamine oxidase (DAO) measured by enzyme-linked immunosorbent assay were used to evaluate the inflammation reactivity as well as the integrity of small intestine. Histological scores were used to assess the mucosal damage, and the C. albicans blood translocation was detected to judge the permeability of intestinal mucosal barrier. Results: The levels of inflammatory factors TNF-alpha., IL-6, and IL-beta in serum and intestine were higher in rats undergone both C. albicans infection and IIRI operation compared with rats in other groups. The levels of DAO (serum: 44.13 +/- 4.30 pg/ml, intestine: 346.21 +/- 37.03 pg/g) and Chiu scores (3.41 +/- 1.09) which reflected intestinal mucosal disruption were highest in group CCI after the operation. The number of C. albicans translocated into blood was most in group CCI ([33.80 +/- 6.60] x 10(2) colony forming unit (CFU)/ml). Conclusion: Intestinal C. albicans infection worsened the IIRI-induced disruption of intestinal mucosal barrier and facilitated the subsequent C. albicans translocation and dissemination.
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