期刊
ANTI-CANCER AGENTS IN MEDICINAL CHEMISTRY
卷 21, 期 3, 页码 316-326出版社
BENTHAM SCIENCE PUBL LTD
DOI: 10.2174/1871520620666200721132522
关键词
Zinc oxide nanoparticle; breast cancer; cytotoxicity; apoptosis; anti-angiogenic effect; anti-oxidant effect
This study evaluated the cytotoxic, apoptotic, anti-angiogenic effects, and gene expression of Zinc Oxide Nanoparticles (ZnO-NPs) synthesized with Carob extracts on human breast cancer cell lines. The results showed that ZnO-NPs could effectively induce apoptosis, inhibit angiogenesis, and have potential as an anticancer agent.
Background: The use of nanoparticles synthesized by the green method to treat cancer is fairly recent. The aim of this study was to evaluate cytotoxicity, apoptotic and anti-angiogenic effects and the expression of involved genes, of Zinc Oxide Nanoparticles (ZnO-NPs) synthesized with Carob extracts on different human breast cancer cell lines. Methods: ZnO-NPs were synthesized using the extracts of Carob and characterized with various analytical techniques. The MCF-7 and MDA-MB231 cells were treated at different times and concentrations of ZnO-NPs. The cytotoxicity, apoptosis, and anti-angiogenic effects were examined using a series of cellular assays. Expression of apoptotic genes (Bax and Bcl2) and anti-angiogenic genes, Vascular Endothelial Growth Factor (VEGF) and its Receptor (VEGF-R) in cancer cells treated with ZnO-NPs were examined with Reverse Transcription quantitative Polymerase Chain Reaction (RT-qPCR). The anti-oxidant activities of ZnO-NPs were evaluated by ABTS and DPPH assay. Results: Exposure of cells to ZnO-NPs resulted in a dose-dependent loss of cell viability. The IC50 values at 24, 48, and 72 hours were 125, 62.5, and 31.2 mu g/ml, respectively (p<0.001). ZnO-NPs treated cells showed, in fluorescent microscopy, that ZnO-NPs are able to upregulate apoptosis and RT-qPCR revealed the upregulation of Bax (p<0.001) and downregulation of Bcl-2 (p<0.05). ZnO-NPs increased VEGF gene expression while decreasing VEGF-R (p<0.001). The anti-oxidant effects of ZnO-NPs were higher than the control group and were dose-dependent (p<0.001). Conclusion: ZnO-NPs synthetized using Carob extract have the ability to eliminate breast cancer cells and inhibit angiogenesis, therefore, they could be used as an anticancer agent.
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