期刊
ANNALS OF SURGICAL ONCOLOGY
卷 27, 期 13, 页码 4950-4960出版社
SPRINGER
DOI: 10.1245/s10434-020-08790-2
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资金
- Comprehensive Cancer Center at Wake Forest Baptist Medical Center's NCI Cancer Center Support Grant [P30CA012197]
- Wake Forest Comprehensive Cancer Center Pilot Fund
- Wake Forest Dean's Hero Award
- Appendix Cancer Pseudomyoxoma Peritonei Foundation (ACPMP)
- North American Organization of Rare Diseases (NORD)
Background Chemotherapy dosing duration and perfusion temperature vary significantly in HIPEC protocols. This study investigates patient-derived tumor organoids as a platform to identify the most efficacious perfusion protocol in a personalized approach. Patients and Methods Peritoneal tumor tissue from 15 appendiceal and 8 colon cancer patients who underwent CRS/HIPEC were used for personalized organoid development. Organoids were perfused in parallel at 37 and 42 degrees C with low- and high-dose oxaliplatin (200 mg/m(2)over 2 h vs. 460 mg/m(2)over 30 min) and MMC (40 mg/3L over 2 h). Viability assays were performed and pooled for statistical analysis. Results An adequate organoid number was generated for 75% (6/8) of colon and 73% (11/15) of appendiceal patients. All 42 degrees C treatments displayed lower viability than 37 degrees C treatments. On pooled analysis, MMC and 200 mg/m(2)oxaliplatin displayed no treatment difference for either appendiceal or colon organoids (19% vs. 25%,p = 0.22 and 27% vs. 31%,p = 0.55, respectively), whereas heated MMC was superior to 460 mg/m(2)oxaliplatin in both primaries (19% vs. 54%,p < 0.001 and 27% vs. 53%,p = 0.002, respectively). In both appendiceal and colon tumor organoids, heated 200 mg/m(2)oxaliplatin displayed increased cytotoxicity as compared with 460 mg/m(2)oxaliplatin (25% vs. 54%,p < 0.001 and 31% vs. 53%,p = 0.008, respectively). Conclusions Organoids treated with MMC or 200 mg/m(2)heated oxaliplatin for 2 h displayed increased susceptibility in comparison with 30-min 460 mg/m(2)oxaliplatin. Optimal perfusion protocol varies among patients, and organoid technology may offer a platform for tailoring HIPEC conditions to the individual patient level.
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