期刊
ANNALS OF BIOMEDICAL ENGINEERING
卷 49, 期 1, 页码 394-406出版社
SPRINGER
DOI: 10.1007/s10439-020-02560-7
关键词
Polymeric nerve conduit; Physical guidance cue; Groove texture; Peripheral nerve regeneration; Dry-jet wet spinning
资金
- National Key Research and Development Program of China [2018YFA0703000]
- Key Research and Development Program of Zhejiang Province [2017C01063]
- Science Fund for Creative Research Groups of the National Natural Science Foundation of China [51821093]
- National Natural Science Foundation of China [U1609207, 81873911]
- Public Projects of Zhejiang Province [2019C03033]
- Zhejiang Medical Science and Technology Project [2019KY569]
- Natural Foundation of Ningbo [2017A610215]
The study illustrates that nerve conduits with micro-grooved inner textures significantly improve peripheral nerve regeneration, leading to enhanced nerve tissue repair efficiency. These conduits show similar repair capacity to autografts, demonstrating the potential for improved nerve tissue engineering strategies.
Physical guidance cues play an important role in enhancing the efficiency of nerve conduits for peripheral nerve injury repair. However, very fewin vivoinvestigations have been performed to evaluate the repair efficiency of nerve conduits with micro-grooved inner textures. In this study, polyacrylonitrile nerve conduits were prepared using dry-jet wet spinning, and micro-grooved textures were incorporated on the inner surface. The nerve conduits were applied to treat 10 mm sciatic nerve gaps in Sprague-Dawley (SD) rats. Sixteen weeks following implantation, nerve function was evaluated based on heat sensory tests, electrophysiological assessments and gastrocnemius muscle mass measurements. The thermal latency reaction and gastrocnemii weight of SD rats treated with grooved nerve conduits were almost 25% faster and 60% heavier than those of SD rats treated with smooth nerve conduits. The histological and immunohistochemical stain analyses showed the repair capacity of inner grooved conduits was found to be similar to that of autografts. These results suggest that grooved nerve conduits with groove width larger than 300 mu m significantly improve peripheral nerve regeneration by introducing physical guidance cues. The obtained results can support the design of nerve conduits and lead to the improvement of nerve tissue engineering strategies.
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