期刊
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
卷 59, 期 42, 页码 18619-18626出版社
WILEY-V C H VERLAG GMBH
DOI: 10.1002/anie.202005505
关键词
DNA lattices; Holliday junctions; host-guest scaffolds; self-assembly; structural DNA nanotechnology
资金
- Howard Hughes Medical Institute
- Department of Energy Office of Science User Facility [DE-AC02-05CH11231]
- U.S. Department of Energy, Office of Biological and Environmental Research [DE-AC02-06CH11357]
- DOE Office of Science [DE-SC0012704]
- National Institute of Health, National Institute of General Medical Sciences (NIGMS) through a Biomedical Technology Research Resource P41 grant [P41GM111244]
- Arizona State University
- Air Force Office of Scientific Research [FA9550-17-1-0053]
- National Science Foundation Division of Materials Research [NSF2004250]
- DOE Office of Biological and Environmental Research [KP1605010]
- Presidential Strategic Initiative Fund from Arizona State University
DNA is an ideal molecule for the construction of 3D crystals with tunable properties owing to its high programmability based on canonical Watson-Crick base pairing, with crystal assembly in all three dimensions facilitated by immobile Holliday junctions and sticky end cohesion. Despite the promise of these systems, only a handful of unique crystal scaffolds have been reported. Herein, we describe a new crystal system with a repeating sequence that mediates the assembly of a 3D scaffold via a series of Holliday junctions linked together with complementary sticky ends. By using an optimized junction sequence, we could determine a high-resolution (2.7 angstrom) structure containing R3 crystal symmetry, with a slight subsequent improvement (2.6 angstrom) using a modified sticky-end sequence. The immobile Holliday junction sequence allowed us to produce crystals that provided unprecedented atomic detail. In addition, we expanded the crystal cavities by 50 % by adding an additional helical turn between junctions, and we solved the structure to 4.5 angstrom resolution by molecular replacement.
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