期刊
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
卷 59, 期 47, 页码 20754-20766出版社
WILEY-V C H VERLAG GMBH
DOI: 10.1002/anie.202005398
关键词
analytical biochemistry; biosensors; CRISPR; in vitro diagnostics; in vivo biosensing
资金
- ARC Future Fellowship [FT160100039]
- UNSW Digital Grid Futures Institute Seed Fund
- UNSW Biomedical Engineering Seed Fund
- UNSW-CAS Collaborative Research Seed Fund Program [172644KYSB20190059]
Recent advances in CRISPR based biotechnologies have greatly expanded our capabilities to repurpose CRISPR for the development of biomolecular sensors for diagnosing diseases and understanding cellular pathways. The key attribute that allows CRISPR to be widely utilized is the programmable and highly selective mechanism. In this Minireview, we first illustrate the molecular principle of CRISPR functioning process from sensing to actuating. Next, the CRISPR based biosensing strategies for nucleic acids, proteins and small molecules are summarized. We highlight some of recent advances in applications for in vitro detection of biomolecules and in vivo imaging of cellular networks. Finally, the challenges with, and exciting prospects of, CRISPR based biosensing developments are discussed.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据