期刊
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
卷 59, 期 38, 页码 16366-16370出版社
WILEY-V C H VERLAG GMBH
DOI: 10.1002/anie.202004447
关键词
biohybrids; DNA catenane walkers; DNA nanotechnology; molecular machines
资金
- Max-Planck-Society
- University of Bonn through a Max-Planck-Fellowship
- Alexander von Humboldt Foundation
- Projekt DEAL
DNA walkers are molecular machines that can move with high precision onthe nanoscale due to their structural and functional programmability. Despite recent advances in the field that allow exploring different energy sources, stimuli, and mechanisms of action for these nanomachines, the continuous operation and reusability of DNA walkers remains challenging because in most cases the steps, once taken by the walker, cannot be taken again. Herein we report the path regeneration of a burnt-bridges DNA catenane walker using RNase A. This walker uses a T7RNA polymerase that produces long RNA transcripts to hybridize to the path and move forward while the RNA remains hybridized to the path and blocks it for an additional walking cycle. We show that RNA degradation triggered by RNase A restores the path and returns the walker to the initial position. RNase inhibition restarts the function of the walker.
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