期刊
ACTA PHARMACOLOGICA SINICA
卷 42, 期 4, 页码 633-640出版社
NATURE PUBL GROUP
DOI: 10.1038/s41401-020-0465-8
关键词
PGK1; NG52; kinase inhibitor; Warburg effect; glioma
资金
- National Natural Science Foundation of China [81773777, 81673469, 81872748, 81803366]
- National Key Research and Development Program of China [2016YFA0400900]
- Natural Science Foundation of Anhui Province [1908085QH348]
- Science and Technology Major Projects of Anhui Province [17030801025]
- China Postdoctoral Science Foundation [2018T110634, 2018M630720, 2019M652057]
- Frontier Science Key Research Program of CAS [QYZDB-SSW-SLH037]
- CASHIPS Director's Fund [BJPY2019A03]
- Key Program of 13th FiveYear Plan of CASHIPS [KP-2017-26]
- National Program for Support of Top-Notch Young Professionals
Inhibition of PGK1 kinase activity may be a potential strategy for glioma treatment, as demonstrated by NG52 reversing the Warburg effect and switching cellular glucose metabolism mode.
Inhibition of glycolysis process has been an attractive approach for cancer treatment due to the evidence that tumor cells are more dependent on glycolysis rather than oxidative phosphorylation pathway. Preliminary evidence shows that inhibition of phosphoglycerate kinase 1 (PGK1) kinase activity would reverse the Warburg effect and make tumor cells lose the metabolic advantage for fueling the proliferation through restoration of the pyruvate dehydrogenase (PDH) activity and subsequently promotion of pyruvic acid to enter the Krebs cycle in glioma. However, due to the lack of small molecule inhibitors of PGK1 kinase activity to treat glioma, whether PGK1 could be a therapeutic target of glioma has not been pharmacologically verified yet. In this study we developed a high-throughput screening and discovered that NG52, previously known as a yeast cell cycle-regulating kinase inhibitor, could inhibit the kinase activity of PGK1 (the IC50 = 2.5 +/- 0.2 mu M). We showed that NG52 dose-dependently inhibited the proliferation of glioma U87 and U251 cell lines with IC(50)values of 7.8 +/- 1.1 and 5.2 +/- 0.2 mu M, respectively, meanwhile it potently inhibited the proliferation of primary glioma cells. We further revealed that NG52 (12.5-50 mu M) effectively inhibited the phosphorylation of PDHK1 at Thr338 site and the phosphorylation of PDH at Ser293 site in U87 and U251 cells, resulting in more pyruvic acid entering the Krebs cycle with increased production of ATP and ROS. Therefore, NG52 could reverse the Warburg effect by inhibiting PGK1 kinase activity, and switched cellular glucose metabolism from anaerobic mode to aerobic mode. In nude mice bearing patient-derived glioma xenograft, oral administration of NG52 (50, 100, 150 mg center dot kg(-1)center dot d(-1), for 13 days) dose-dependently suppressed the growth of glioma xenograft. Together, our results demonstrate that targeting PGK1 kinase activity might be a potential strategy for glioma treatment.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据