期刊
ACS NANO
卷 14, 期 7, 页码 8157-8170出版社
AMER CHEMICAL SOC
DOI: 10.1021/acsnano.0c01486
关键词
phototherapy; immunotherapy; antibacterial; MRSA; biofilm infection
类别
资金
- National Science Fund for Distinguished Young Scholars [51925104]
- National Natural Science Foundation of China [51671081, 51871162]
- NSFC key program [51631007]
- Natural Science Fund of Hubei Province [2018CFA064]
- Hong Kong ITC [ITS/287/17, GHX/002/14SZ]
- Health and Medical Research Fund [03142446]
Clinically, methicillin-resistant Staphylococcus aureus (MRSA) biofilm infection inevitably induces the failure of bone implants. Herein, a hydrophilic and viscous hydrogel of poly(vinyl alcohol) modified with chitosan, polydopamine, and NO release donor was formed on a red phosphorus nanofilm deposited on a titanium implant (Ti-RP/PCP/RSNO). Under the irradiation of near-infrared light (NIR), peroxynitrite (center dot ONOO-) was formed by the reaction between the released NO and superoxide (center dot O-2(-)) produced by the RP nanofilm. Specifically, we revealed the antibacterial mechanism of the ONOO- against the MRSA biofilm. In addition, osteogenic differentiation was promoted and inflammatory polarization was regulated by the released NO without NIR irradiation through upregulating the expression of Opn and Ocn genes and TNF-alpha. The MRSA biofilm was synergistically eradicated by center dot ONOO-, hyperthermia, and center dot O2- under NIR irradiation as well as the immunoreaction of the M1 polarization. The in vivo results also confirmed the excellent osteogenesis and biofilm eradication by released NO from the RP/PCP/RSNO system under NIR irradiation, indicating the noninvasive tissue reconstruction of MRSA-infected tissues through phototherapy and immunotherapy.
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