期刊
ACS APPLIED MATERIALS & INTERFACES
卷 12, 期 33, 页码 36873-36881出版社
AMER CHEMICAL SOC
DOI: 10.1021/acsami.0c08722
关键词
dually crosslinked supramolecular hydrogel; sensor; lysophosphatidic acid; SPR
资金
- National Natural Science Foundation of China [51903014, 21774007]
- Beijing Natural Science Foundation [2202043]
- China Postdoctoral Science Foundation [2019 M660414]
Lysophosphatidic acid (LPA) as the biomarker of early stage ovarian cancer is essentially difficult to detect due to lack of target spots. A dually crosslinked supramolecular hydrogel (DCSH) was developed to achieve sensing of LPA, which acts as a competitive guest molecule triggering the responsive crosslinking of the DCSH. Through this strategy, the surface plasmon resonance combined with optical waveguide spectroscopy could be used to quantitatively detect LPA with a responsive range covering physiological conditions (in pure form as well as mimicking LPA plasma solution) with high selectivity and sensitivity. LPA efficiently immerses into the host molecule beta-cyclodextrin (beta-CD) up to a 1:2 ratio by the competitive interaction mechanism, confirmed by one-dimensional nuclear overhauser effect spectroscopy (1D NOESY), high-resolution mass spectrometry (HRMS), isothermal titration calorimetry (ITC), and computational simulation. Our method opens a new strategy to detect biomarkers without target spots and provides a platform for surface plasmon resonance (SPR)-based sensors measuring small molecules.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据