Agaricus bisporus mediated biosynthesis of copper nanoparticles and its biological effects: An in-vitro study

Biofabrication of Copper nanoparticles (CuNPs) using Agaricus bisporus aqueous extract and its potential in-vitro biological activities were attempted in the present work. The synthesized CuNPs was confirmed by absorption peak at 551 nm using UV-Vis spectroscopy. The crystalline properties and the biofunctional groups involved in the reduction process were authenticated by XRD and FTIR spectroscopic techniques. Zeta potential value of -40 mV confirmed the stability of CuNPs. SEM and TEM analysis revealed agglomerated morphology and the presence of elemental Cu was confirmed by EDAX. In-vitro anti-bacterial assay demonstrated good bactericidal effects with more inhibitory action against Enterobacter aerogens (15 +/- 0.08 mm). Anti-oxidant assays revealed potential scavenging effect against ABTS radical (82%); NO radical (76%) and DPPH radical (72%). Cytotoxic assay also revealed potential inhibitory effects against human colon cancer cell lines (SW620) with 60% cell viability recorded at 500 mu g/ml concentration. Fluorescent microscopic observation of SW620 cells stained by DCFHDA and acridine orange dye displayed fragmentation, membrane loss and generation of ROS apoptotic features.