期刊
FOODS
卷 9, 期 3, 页码 -出版社
MDPI
DOI: 10.3390/foods9030305
关键词
lead (II); ELISA; monoclonal antibody (mAb); isothiocyanobenzyl-EDTA (ITCBE); chemiluminescent enzyme immunoassay (CLEIA)
资金
- National Natural Science Foundation of China [31572556, 31873006]
- Key Program for International S&T Cooperation Project of Shaanxi Province [2017KW-ZD-10]
- Incubation Project on State Key Laboratory of Biological Resources and Ecological Environment of Qinba Areas [SLGPT2019KF04-04]
Lead is a heavy metal with increasing public health concerns on its accumulation in the food chain and environment. Immunoassays for the quantitative measurement of environmental heavy metals offer numerous advantages over other traditional methods. ELISA and chemiluminescent enzyme immunoassay (CLEIA), based on the mAb we generated, were developed for the detection of lead (II). In total, 50% inhibitory concentrations (IC50) of lead (II) were 9.4 ng/mL (ELISA) and 1.4 ng/mL (CLEIA); the limits of detection (LOD) were 0.7 ng/mL (ic-ELISA) and 0.1 ng/mL (ic-CLEIA), respectively. Cross-reactivities of the mAb toward other metal ions were less than 0.943%, indicating that the obtained mAb has high sensitivity and specificity. The recovery rates were 82.1%-108.3% (ic-ELISA) and 80.1%-98.8% (ic-CLEIA), respectively. The developed methods are feasible for the determination of trace lead (II) in various samples with high sensitivity, specificity, fastness, simplicity and accuracy.
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