期刊
FOODS
卷 9, 期 4, 页码 -出版社
MDPI
DOI: 10.3390/foods9040474
关键词
non-heme iron; plant-based food; Mediterranean and middle-eastern cuisine; hummus; food processing and formulation; in vitro digestion; iron dialysis; iron uptake by Caco-2 cells
资金
- Regional Council of Bourgogne-Franche-Comte
- European Regional Development Fund (FEDER)
Hummus, an iron-containing plant-based dish mainly made from chickpea puree, tahini, lemon juice and garlic, could be a valuable source of iron when bioavailable. Since the processing and formulation of food influence iron bioavailability, the present study investigated for the first time, their effects on hummus. Firstly, iron bioaccessibility was assessed on eight samples (prepared according to the screening Hadamard matrix) by in vitro digestion preceding iron dialysis. Then, iron bioavailability of four selected samples was estimated by the in vitro digestion/Caco-2 cell model. Total and dialyzable iron were determined by the atomic absorption spectrometry and ferritin formation was determined using an ELISA kit. Only autoclaving, among other processes, had a significant effect on iron bioaccessibility (+9.5, p < 0.05). Lemon juice had the highest positive effect (+15.9, p < 0.05). Consequently, the effect of its acidic components were investigated based on a full factorial 2(3) experimental design; no significant difference was detected. Garlic's effect was not significant, but tahini's effect was negative (-8.9, p < 0.05). Despite the latter, hummus had a higher iron bioavailability than only cooked chickpeas (30.4 and 7.23 ng ferritin/mg protein, respectively). In conclusion, hummus may be a promising source of iron; further in vivo studies are needed for confirmation.
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