期刊
MICROORGANISMS
卷 8, 期 5, 页码 -出版社
MDPI
DOI: 10.3390/microorganisms8050711
关键词
Talaromyces albobiverticillius 30548; submerged fermentation; pigments; biomass; optimization; Box-Behnken experimental design; response surface modeling
类别
资金
- Conseil Regional de La Reunion, Reunion Island, France [DIRED 20140704, 2016020577/181733]
Talaromyces albobiverticillius 30548 is a marine-derived pigment producing filamentous fungus, isolated from the La Reunion island, in the Indian Ocean. The objective of this study was to examine and optimize the submerged fermentation (SmF) process parameters such as initial pH (4-9), temperature (21-27 degrees C), agitation speed (100-200 rpm), and fermentation time (0-336 h), for maximum production of pigments (orange and red) and biomass, using the Box-Behnken Experimental Design and Response Surface Modeling (BBED and RSM). This methodology allowed consideration of multifactorial interactions between a set of parameters. Experiments were carried out based on the BBED using 250 mL shake flasks, with a 100 mL working volume of potato dextrose broth (PDB). From the experimental data, mathematical models were developed to predict the pigments and biomass yields. The individual and interactive effects of the process variables on the responses were also investigated (RSM). The optimal conditions for maximum production of pigments and biomass were derived by the numerical optimization method, as follows-initial pH of 6.4, temperature of 24 degrees C, agitation speed of 164 rpm, and fermentation time of 149 h, respectively.
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