期刊
MICROORGANISMS
卷 8, 期 4, 页码 -出版社
MDPI
DOI: 10.3390/microorganisms8040529
关键词
Docosahexaenoic acid (DHA); polyunsaturated fatty acids (PUFAs); mutant strain; Aurantiochytrium sp; transcriptome
类别
资金
- National Key Research and Development Project [2018YFA0902500]
- Natural Science Foundation of Guangdong Province [2018A030313139]
- Joint R&D Project of Shenzhen-Hong Kong Innovation [SGLH20180622152010394]
- Hong Kong Innovation and Technology Commission TCFS [GHP/087/18SZ]
- Shenzhen Taifeng East Marine Biotechnology Co. Ltd
- Natural Science Foundation of Shenzhen [KQJSCX20180328093806045]
- Shenzhen science and technology application demonstration project [KJYY20180201180253571]
- Shenzhen peacock plan [827-000192]
Docosahexaenoic acid (DHA), a n-3 long-chain polyunsaturated fatty acid, is critical for physiological activities of the human body. Marine eukaryote Aurantiochytrium sp. is considered a promising source for DHA production. Mutational studies have shown that ultraviolet (UV) irradiation (50 W, 30 s) could be utilized as a breeding strategy for obtaining high-yield DHA-producing Aurantiochytrium sp. After UV irradiation (50 W, 30 s), the mutant strain X2 which shows enhanced lipid (1.79-fold, 1417.37 mg/L) and DHA (1.90-fold, 624.93 mg/L) production, was selected from the wild Aurantiochytrium sp. Instead of eicosapentaenoic acid (EPA), 9.07% of docosapentaenoic acid (DPA) was observed in the mutant strain X2. The comparative transcriptomic analysis showed that in both wild type and mutant strain, the fatty acid synthesis (FAS) pathway was incomplete with key desaturases, but genes related to the polyketide synthase (PKS) pathway were observed. Results presented that mRNA expression levels of CoAT, AT, ER, DH, and MT down-regulated in wild type but up-regulated in mutant strain X2, corresponding to the increased intercellular DHA accumulation. These findings indicated that CoAT, AT, ER, DH, and MT can be exploited for high DHA yields in Aurantiochytrium.
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