期刊
BLOOD
卷 125, 期 15, 页码 2349-2358出版社
AMER SOC HEMATOLOGY
DOI: 10.1182/blood-2014-09-599423
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类别
资金
- AIRC [IG-13018]
- Special Grant 5xmille [9962]
- My first AIRC grant [15925]
- Ministero della Salute [RF-2010-2308270, RF-2010-2316606]
- Grant 5xmille
- Ricerca Corrente
- Ministero dell'Istruzione, Universita e Ricerca (Grant Progetto di Rilevante Interesse Nazionale, PRIN)
We prospectively assessed functional and phenotypic characteristics of gamma delta T lymphocytes up to 7 months after HLA-haploidentical hematopoietic stem cell transplantation (haplo-HSCT) depleted of alpha beta(+) T cells and CD19(+) B cells in 27 children with either malignant or nonmalignant disorders. We demonstrate that (1) gamma delta T cells are the predominant T-cell population in patients during the first weeks after transplantation, being mainly, albeit not only, derived from cells infused with the graft and expanding in vivo; (2) central-memory cells predominated very early posttransplantation for both V delta 1 and V delta 2 subsets; (3) V delta 1 cells are specifically expanded in patients experiencing cytomegalovirus reactivation and are more cytotoxic compared with those of children who did not experience reactivation; (4) these subsets display a cytotoxic phenotype and degranulate when challenged with primary acute myeloid and lymphoid leukemia blasts; and (5) V delta 2 cells are expanded in vitro after exposure to zoledronic acid (ZOL) and efficiently lyse primary lymphoid and myeloid blasts. This is the first detailed characterization of gamma delta T cells emerging in peripheral blood of children after CD19(+) B-cell and alpha beta(+) T-cell-depleted haplo-HSCT. Our results can be instrumental to the development of clinical trials using ZOL for improving gamma delta T-cell killing capacity against leukemia cells.
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