期刊
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
卷 8, 期 -, 页码 -出版社
FRONTIERS MEDIA SA
DOI: 10.3389/fcell.2020.00068
关键词
3D culture; fibroblast growth factor 2; involution; lactation; mammary gland; milk production; organoid; prolactin
资金
- Pasteur, Centre National pour la Recherche the Agence Nationale de la Recherche [ANR-10-LABX-73, ANR-16-CE13-0017-01]
- Fondation ARC [PJA 20161205028, 20181208231]
- AFM-Telethon Foundation
- Grant Agency of Masaryk University [MUNI/G/1446/2018]
- Revive Consortium
- P-Pool (Masaryk University, Faculty of Medicine), Amgen Scholar Europe program
- P-Pool (Masaryk University, Faculty of Medicine), Amgen Scholar Erasmus C program
- Sorbonne Universite
- Agence Nationale de la Recherche (ANR) [ANR-16-CE13-0017] Funding Source: Agence Nationale de la Recherche (ANR)
Mammary gland development occurs mainly after birth and is composed of three successive stages: puberty, pregnancy and lactation, and involution. These developmental stages are associated with major tissue remodeling, including extensive changes in mammary epithelium, as well as surrounding stroma. Three-dimensional (3D) mammary organoid culture has become an important tool in mammary gland biology and enabled invaluable discoveries on pubertal mammary branching morphogenesis and breast cancer. However, a suitable 3D organoid model recapitulating key aspects of lactation and involution has been missing. Here, we describe a robust and straightforward mouse mammary organoid system modeling lactation and involution-like process, which can be applied to study mechanisms of physiological mammary gland lactation and involution as well as pregnancy-associated breast cancer.
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